Comparison of a PCR-based diagnostic assay for <i>Mycoplasma pulmonis</i> with traditional detection techniques
نویسندگان
چکیده
Current diagnosis of infection by Mycoplasma pulmonis, an important pathogen of laboratory rodent colonies worlwide, is based on serological, histopathological and culture techniques which can be slow and unreliable. A polymerase chain reaction (PCR) assay for M. pulmonis diagnosis was compared to current diagnostic methods. This PCR based technique allows a more specific, sensitive and rapid diagnosis of M. pulmonis from various tissues by comparison with culture and histopathology.
منابع مشابه
Comparison of a PCR-based diagnostic assay for Mycoplasma pulmonis with traditional detection techniques.
Current diagnosis of infection by Mycoplasma pulmonis, an important pathogen of laboratory rodent colonies worldwide, is based on serological, histopathological and culture techniques which can be slow and unreliable. A polymerase chain reaction (PCR) assay for M. pulmonis diagnosis was compared to current diagnostic methods. This PCR based technique allows a more specific, sensitive and rapid ...
متن کاملDetection of Mycoplasma pulmonis in experimentally infected laboratory rats by 16S rRNA amplification.
Recently, an rRNA-based polymerase chain reaction (PCR) has been developed for the detection of murine mycoplasmas at both the genus and species level (F. J. M. van Kuppeveld, J. T. M. van der Logt, A. F. Angulo, M. J. van Zoest, W. G. V. Quint, H. G. Niesters, J. M. D. Galama, and W. J. G. Melchers, Appl. Environ. Microbiol. 58:2606-2615, 1992). In this study, the diagnostic value of this PCR ...
متن کاملEvaluation of Mycoplasma Muris and Pulmonis infections in Genital and Respiratory Tracts
Abstract Mycoplasma pulmonis and muris infections have been associated with several diseases in conventionally housed laboratory rat and mice colonies. In naturally infected of mycoplasma pulmonis in mice and rat colonies, the respiratory organ appears to be the favored site of colonization; so, it was not surprising to see that infection spread from the site of urogenital tract to the respirat...
متن کاملApplication of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms
Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...
متن کاملPolymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum
Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes, and also the smallest organisms that can live independently. They are able to cause serious and chronic disease because of some unique characteristics. Mycoplasma gallisepticum (MG) is an important avian pathogen causing significant economical losses within the poultry industry. The...
متن کامل